Conversion of Hi-C matrices of different file formats. We support the conversion of hic to cool format via hic2cool, and homer, HicPro, h5 and cool format to h5, cool, homer or ginteractions format. Moreover, hicConvertFormat accepts multiple input files from one format with different resolutions and creates a mcool file. Each original file is stored under the path e.g. ::/resolutions/10000. A batch computation is possible, the number of input files and output files needs to match, all input files need to be of the same format type and all output files too. For input and output of cooler files special options are available, for all other formats they will be ignored.HiCPro file format needs an additional bed file as input.
Download Hi Converter For Mac 1.2 Pro
input file(s). Could be one or many files.
- Gen 1 2 3 Pokemon Editor and Converter for Windows + Mac OS X. Becouse i download the app and try out but all the time says to me that i need java.the problem i.
- Safety instructions for electromagnetic fields (EMF) WARNING. Danger to life from electromagnetic fields. Electromagnetic fields (EMF) are generated by the operation of electrical power equipment such as transformers, converters or motors. People with pacemakers or implants are at a special risk in the immediate vicinity of these.
Download Camino. A listing of all available releases of Camino. Version 2.1 For Mac OS X 10.4 or later. Camino 2.1.2 is the latest stable release of Camino. For more information about Camino 2.1.2, please see our release notes. English Edition Supports US, British, and International English; Multilingual Edition. Handbrake for Windows is a free and open-source program that allows you to convert video files to a wide range of formats using several post-production tools and presets. With Handbrake, you can encode video files and convert them to work on your smartphone, home media player, or upload to a video hosting site like Vimeo and YouT.
File name to save the exported matrix.
Possible choices: h5, cool, hic, homer, hicpro, 2D-text
File format of the input matrix file.The following options are available: h5 (native HiCExplorer format based on hdf5 storage format), cool, hic, homer, hicpro, 2D-text.
Possible choices: cool, h5, homer, ginteractions, mcool, hicpro
Output format. The following options are available: h5 (native HiCExplorer format based on hdf5 storage format). cool, ginteractions, homer, mcool and hicpro (Default: “cool”).
Name of the column which stores the correction factors. The information about the column names can be figured out with the tool hicInfo. Option only for cool input files (Default: “weight”).
If set, division is applied for correction. Default is a multiplication. Option only for cool input files.
Store the applied correction and do not set correction factors. Option only for cool input files.
Load only one chromosome. Option only for cool input files.
Enforce datatype of counts to integer. Option only for cool input files.
Download HiConverter For Mac 1.2
Load only ‘count’ data and do not apply a correction. Option only for cool input files.
List of resolutions that should be added.
This option is for the input format 2D-text only and will be ignored else.File with the chromosome sizes for your genome. A tab-delimited two column layout “chr_name size” is expectedPlease consider that this option causes that only reads are considered which are on the listed chromosomes.Use this option to guarantee fixed sizes. An example file is available via UCSC: http://hgdownload.soe.ucsc.edu/goldenPath/dm3/bigZips/dm3.chrom.sizes
show program’s version number and exit
Bed file(s) of hicpro file format.
To reproduce analyses and to compare and use different Hi-C analysis software, conversion between interaction matrix file formats is crucial.However, most Hi-C softwares are only supporting their own data format which makes the exchange difficult. HiCExplorer supports a range ofinteraction matrices, both for import and for export.
With HiCExplorer version 2.2 hicConvertFormat and hicAdjustMatrix replace hicExport from HiCExplorer 2.1 and older versions.
HiCExplorer uses the library hic2cool to convert .hic interaction matrix files to the cool format. Usually .hic fileshave the three correction factors KR, VC or VC_SQRT; however these cannot be applied natively by HiCExplorer tools becauseHiCExplorer expects the correction values to be stored in the column weight.To work with corrected data the correction factors need to applied separately, see section cool to cool.
The following example will convert a hic file which contains the resolution of 1000 to a cool file with 10kb resolution. The desiredresolution needs to be existing in the hic file. If no resolution parameter is defined, a mcool file with all available resolutions is created.
It is only possible to convert from hic to cool format, no other formats are supported.
cool to cool¶
The cool file format is developed and maintained by the Mirny lab and allows to access interaction matrices in an easy to use data format.
The cool data format allows to use the following options:
correction_name: In case correction factors are not stored in ‘weight’ the correct column name can be defined using this parameter and the resulting matrix will store the values in ‘weight’.
correction_division: Correction factors can be applied by a multiplication or a division. The default behaviour is to use the multiplication, in case the correction factors are inverted, set this parameter.
store_applied_correction: Set this parameter if correction factors should be applied on the data and should be written back to colum ‘counts’ in the corrected form and not as raw. Default: not set.
chromosomes: Define a list of chromosomes which should be included in the output matrix. All chromosomes which are not defined are not part of the new matrix. This parameter can speed up the processing especially if only one chromosome is used.
enforce_integer: Raw interaction data is stored as integers, after the correction is applied the data is a float. Set a this parameter to enforce integer values in the new matrix.
load_raw_values: Set this parameter if the interaction data should not be loaded with the correction factors.
Homer is a software for ‘motif discovery and next generation sequencing analysis’ and supports Hi-C. HiCExplorer is able to read and write the interaction matrix from Homer. Homer stores the interaction matrix in a simple text file as a dense matrix. To writelarge matrices in Homer format needs a lot of space and can take a few ours to days.
HiC-Pro file format needs an additional bed file as input:
Download Hiconverter For Mac 1.2 Full
Create a mcool file¶
With HiCExplorer it is possible to create a multiple cool (mcool) file. These mcool files can be used e.g. with HiGlass.
To create an mcool file, use as input either one matrix in one of the supported read formats and define the desired resolutions or definemultiple input matrices. In the second case, the matrices should all have different resolutions.
The resolutions need to be a multiple of the input matrix i.e. matrix with 10kb, 20kb and 30kb are possible but not 35kb.
Download Hiconverter For Mac 1.2 Free
The mcool matrix contains the individual matrices as follows: